CHEM2208 Laboratory Report

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CHEM2208 Laboratory Report

Practical 1: Plasmid DNA purification and E. coli transformation

Pre-Lab Questions

1. What is a “miniprep” (1 mark)

2. What are competent cells (1 mark)

3. Draw the chemical structure of IPTG. (1 mark)

4. Why is ampicillin added to the LB medium (1 mark)

5. What is a plasmid (1 mark)

Results

1. Briefly describe the experiments. (3 marks)

2. What is the DNA concentration of the miniprep (1 mark)

3. What evidence for the purity of the plasmid preparation do you have (2 marks)

4. What is the number of colonies after transformation (include photograph) (2 marks)

5. Calculate the transformation efficiency CFU/μg of DNA. (2 marks)

Post-Lab Questions

1. Describe the central dogma of molecular biology. (2 marks)

2. Draw a schematic sketch of a pET overexpression vector, highlighting its essential parts at the right position. (3 marks)